Abstract: In order to transplant the winter wheat seedling germinated from immature embryo to local field in summer, this experiment employed three different aged immature embryos (11- 13, 14- 16, 17- 19 days) of‘Jimai 20’,‘Yanyou 361’,‘Tainong 18’,‘Zhongyou 9507’and (Jimai 20×Yanyou 361) F1, which separately inoculated on three types of media (MB, A and B), to carry out the study about immature embryo one- step seedling formation through in vitro culture. The results showed that between nine kinds of inoculation process, the 11 to 13 day- old immature embryo inoculated in A medium showed the highest rate of germination (98.30% on average), the 14 to 16 day-old immature embryo inoculated in MB medium also got higher rate of germination (95.80% on average), the 17 to 19 day-old immature embryo inoculated in B medium showed the rate of germination as 95.20% on average, while the 17 to 19 day- old immature embryo inoculated in MB medium got the lowest rate of germination (16.20% on average). The 14 to 16 day- old immature embryo inoculated in MB medium not only showed the higher rate of germination, but also had longer root, more lateral roots, which indicated that MB medium was suitable for 14 to 16 day-old immature embryo.