Abstract: The aim was to establish a method for determining nickel content in the tanichthys albonubes feed. By flame atomic absorption spectrometer establish a simple, rapid and accurate determination of nickel content in feed method, using microwave digestion, dry digestion and wet digestion three kinds of pretreatment method combine flame atomic absorption spectrometry to determine nickel content in feed. Comparative analysis to establish the optimum conditions for nickel, and various interference factors were integrated consideration. The results showed that, flame atomic absorption spectrometry determination of nickel standard curve r=0.9999, and the detection limit was 0.012μg/mL; TritonX- 100- ethanol as a matrix modifier; using microwave digestion, dry and wet digestion digestion process albonubes feed samples, the measured average nickel content of 6.05, 5.76, 5.98 mg/kg, three kinds of pretreatment methods determination of nickel showed no significant difference; precision experiment relative standard deviations were 3.30% , 4.86% and 4.96%, and the relative standard deviations were less than 5%; three kinds of pretreatment average recoveries were 98.3%, 94.4% and 97.1% recovery relative standard deviation of 3.3% or less. Three method is simple, rapid, accurate measurement results are satisfactory, can be used for the determination of nickel content in feed.