Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (20): 11-16.doi: 10.11924/j.issn.1000-6850.2013-3397

Special Issue: 生物技术 畜牧兽医

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Expression of Bovine IL-6 and the Adjuvant Effects on Subunit Vaccine of Foot-and-Mouth Virus

  

  • Received:2013-12-30 Revised:2014-03-01 Online:2014-07-15 Published:2014-07-15

Abstract: To detect the adjuvant effects of IL-6 on subunit vaccine VP1 of foot-and-mouth disease, bovine IL- 6 and VP1 sequences were cloned and inserted into the expressing vector pGEX- 6p- 1 respectively. Recombined plasmids pGEX- 6P- 1/BoIL- 6, pGEX- 6P- 1/VP1 and pGEX- 6P- 1/BoIL- 6/VP1 were constructed and expressed in E. coli RS21 with a high expression level. The IL-6 effects on immune response in guinea pig as molecule adjuvant were detected. The results showed recombined plasmids were expressed correctly in E. coli in inclusion body. After recombined proteins immunized Guinea pigs for three times, IL-6/ VP1 group induced a high level of humoral immune response and cellular immune response, which had a similar immune level to vaccine group and VP1+CSF group. The results indicated IL-6 could enhance the immunological reaction level as molecule adjuvant.