Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (16): 170-174.doi: 10.11924/j.issn.1000-6850.2013-3406

Special Issue: 园艺

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Study on Separation and Purification of Total Flavonoids in Dryopteris fragrans (L.) Schott with AB-8 Macroporous Adsorption Resin

  

  • Received:2013-12-30 Revised:2014-02-27 Online:2014-06-05 Published:2014-06-05

Abstract: The separation and purification conditions of total flavonoids from Dryopteris fragrans (L.) Schott were studied by AB-8 macroporous adsorption resin in this study. Using adsorption rate, elution rate and yield of total flavonoids as indexes to investigate the adsorption and desorption capacity of total flavonoids with AB-8 macroporous adsorption resin. Through the study, the optimum loading concentration of total flavonoids and flow rate were 1.58 mg/mL and 1.5 mL/min respectively. The optimum desorption conditions were achieved by using 70% ethanol as desorption solvent at a flow rate of 1.0 mL/min (2 BV/h). The results showed that AB-8 macrosporus adsorption resin performed perfect adsorption and desorption efficiency. After purification, the purity of total flavonoids was 38.4%.