Abstract: The purpose of this paper was to research the sensibility of the exogenous hormones to the plant height characters and the content of endogenous hormones of‘Ginko leafed’mutation of cucumber, and determine which kind of hormone deficiency belonged to. The mutation and the normal cucumber were used as materials, and the mutation was treated with different contents of exogenous GA₃, IAA. The changes of plant height and internode length of the cucumber were analyzed. Meanwhile, the content of endogenous hormones of cucumbers was determined. The plant height and internode length of the mutation with different treatments were significantly different compared with the normal cucumber. And the content of GA₃ of the mutation was significantly higher than that of the normal cucumber. The results showed that the‘Ginko leafed’mutation of cucumber maybe a gibberellin insensitive mutation.