Abstract: Four kinds of different plant growth regulators with different concentration treatment of DPC、CCC、PP333、 Ethephon on the influence of summer cucumber seedlings biological character, physiological indexes and yield was studied, which adopted the spraying on the surface of the substrate (one time), the substrate surface spraying + cotyledon flattening just open heart leaves show when spraying on the leaf (twice) and soaking seed three kinds of treatments. The results showed that the hypocotyl significantly shortened compare with the control, and leggy cucumber seedling was effectively controlled during the summer with four treatments combination of DPC400ppm(twice), PP33320ppm(one time), CCC400ppm (twice), seed soaking CEPA5000ppm. Compared with the control, Strong seedling index increased, root vigored, increased the rate of the dry matter to the distribution of the root system, improve the quality of the seedling. Cucumber leaf chlorophyll content and disease resistance related enzymes of PPO, POD, CAT activity was enhanced, proline content was increased, ,which enhanced disease resistance of cucumber.