Abstract: In order to scientifically regulate the behavior of the ecotourism resources development and ensure the sustained, healthy and rapid development of the forest ecotourism of the nature reserve. With the Yarlung Zangbo Grand Canyon National Nature Reserve as a study subject, 3 evaluation indices, including the ecotourism resource quality, environmental conditions and development conditions, were chosen as the 1st level indices. 6 evaluation indices, including the natural landscape resources, cultural landscape resources, environmental capacity, environmental quality, geographical features and regional conditions, were chosen as the 2nd level indices. 22 evaluation indices were chosen as the 3rd level indices. Thus a model of hierarchy structure of the ecotourism resource evaluation was established and used to carry out a quantitative evaluation on the Yarlung Zangbo Grand Canyon National Nature Reserve. The evaluation results showed that the score of the natural landscape resources was highest, and then the score of the cultural landscape resources was the second, the evaluation score of the environmental quality was the third, the score of the district characteristics and regional conditions was relatively low in the 2nd level indices. This indicated that natural landscape resources and cultural landscape resources constituted the basic framework for the ecotourism resources of the nature reserve. The score of the resource quality was highest, and then the score of environmental conditions was the second, the evaluation score of the environmental quality was the third, the score of the development conditions was relatively low in the 1st level indices. At last, we can conclude the quantitative evaluation score of tourism resources of the Yarlung Zangbo Grand Canyon National Nature Reserve was 90.6, and the evaluation class was the first class, suggesting that the ecotourism resources of the Yarlung Zangbo Grand Canyon National Nature Reserve is very good, and the development and utilization value should be quite high.