Identification of Trichoderma harzianum TRI2 and Its Biological Control Effect Against Root-knot Nematode

doi:10.11924/j.issn.1000-6850.2014-0337

Abstract

Abstract: In order to control the root-knot nematode disease in the production and provide a theoretical basis for the research and development of a new and efficient bio-nematicide, the Trichoderma spp. was studied. Trichoderma spp. strain TRI2 was identified as Trichoderma harzianum by morphological observation and ITS tef1 sequence analysis. The effects on Meloidogyne incognita J2 were tested by the fermentation broth of biocontrol strain TRI2. And the pot experiment and field experiment were carried out. The results showed that the fermentation liquid of Trichoderma spp. strain TRI2 could kill 100% root-knot nematodes in 48 h, in the pot experiments that the cucumber root-knot loss rate reached 63.5%, control effect on cucumber root knot nematodes was up to 72.2% in the field and had a similar effect to avermectin in the field. The identification and control of strain TRI2 laid the foundation for biocontrol mechanism and its development application.

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