Abstract: In order to study the influence of mulberry active substance (1-NDJ) on the growth and enzymatic activity of 4th S. exigua, which were feed cabbage leaves soaked by different concentrations for 30 second. The results showed that the highest anti-feeding rates were 77.20% and 92.01%, respectively at 24 h and 48 h, and the AFC50 were 0.48 and 0.21 g/L, respectively. The developmental inhibition rates decreased obviously with the increasing 1-DNJ concentration, which could up to 92.34%. While, activity of superoxide dismutase and carboxylesterase of the 4th S. exigua were induced and then inhibited in after treated with 1-DNJ, activity of carboxylesterase decreased significantly than CK (P＜0.05), activity of peroxidase were few changed by 1- DNJ. The action time of anti- feeding and growth inhibitory were logged in effects of protective enzyme system, suggesting that protective enzyme system was sensitive to 1-DNJ. These results implied that mulberry was treasuries of bioactive substances useful as pesticides, which could provide basic data and theoretical basis for development of botanical pesticides using 1-DNJ of mulberry.