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Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (32): 15-20.doi: 10.11924/j.issn.1000-6850.2014-0540

Special Issue: 畜牧兽医

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The Surveillance and Genetic Evolution Analysis of H10 Subtype Avian Influenza Virus in Dongting Lake Region

  

  • Received:2014-03-04 Revised:2014-03-04 Accepted:2014-08-18 Online:2014-11-20 Published:2014-11-20

Abstract: The aim of the study is to understand the distribution and prevalence and genetic evolution of H10 subtype avian influenza virus in the Dongting Lake Region, and propose some measures and reference about the role of avian influenza virus evolution. Duck swabs and environmental swabs were sampled in live poultry wholesale market and waterfowl farm, and avian influenza virus were isolated, and the gene fragments were amplified, sequenced and analyzed by Mega 5 software. Results showed that there were 11 isolates of H10 subtype avian influenza virus in live poultry wholesale market, which were from duck swab. 2 isolates of H10 subtype avian influenza virus was isolated in duck farm. The sequence analysis showed that the cleavage site of HA protein was PELMQGR / GLF, which belonged to a low pathogenic virus, and was in the same branch with H10 subtype avian influenza virus isolated from wild birds in other countries (Mongolia, Sweden, the Netherlands, Thailand, Japan, etc.).