Abstract: To enrich the gene for the newly identified porcine kubovirus, theVP gene of porcine kobuvirus was amplified by RT-PCR method from tissues of piglets associated with diarrhea in Fujian Province and then sequenced, then bioinformatics analysis were conducted to investigate the structure and function of the porcine kubovirus VP gene. The results demonstrated that the porcine kobuvirus VP gene was 2529 bp in length, coding an open reading frame (ORF) with 843 amino acids. The isoelectric point, molecular weight and instability index of porcine kubovirusVP protein were 5.34, 89.603kD, 37.71, respectively. The maximum and the minimum hydrophobicity were 2.522 and - 2.622, respectively. Compared with the PKV VP genes published previously download from GenBank, the sequenced gene shared the highest homogeneity with XX strain, with 86.7%; while had the lowest homogeneity with swine/S-1-HUN/2007/Hungary strain, which was 83.7% . The porcine kubovirus VP gene shares two different phylogenetic relationship branches, and the Chinese porcine kubovirus isolates distribute at both branches.