Abstract: To protect and utilize a precious genetic resource of Youzhou black- skinned Goat, the author conducted experiments to investigate the quality of ram semen. Several semen extenders were studied in order to find out which is the best extender. The results showed that he average ejaculate volume was 0.85 mL, vitality was 81.56% and the rate of terato sperm was 7.27 %, intact acrosome rate was 91.48 %, sperm density was 23.8 billion/mL, all the index met the requirements of GB20557- 2006. The effects of the ram’s spermatozoa conserved inⅢdiluent was better than other diluents，(P＜0.05)．and the effects of the ram’s spermatozoa conserved in low temperature environment is better than in room temperature environment and high temperature. In conclusion, the collected fresh semen of rams in Conservation field of Chongqing city Youyang was found to be of good quality, the rams are capable of hybridization, the diluentⅢcould be used to conserve ram semen .