Effect of Low Phosphorous Stress on Photosynthesis and Chlorophyll Fluorescence Characteristics of Maize Inbred Lines Qi319 and low-phosphorus-tolerant Mutant Qi319-96

doi:10.11924/j.issn.1000-6850.2014-0582

Abstract

Abstract: To better understand the differences on light absorption, distribution and surplus energy dissipation between two different phosphorus efficiency maize genotypes under low phosphorus, ydroponics was conducted with the low phosphorus tolerant mutant Qi319-96 and wild type Qi319 as the test materials. The leaf photosynthesis and chloroplast fluorescence characteristics were detected between two inbred lines grown under P-deficient (KH2PO4 5 μmol/L, -P) and P-sufficient (KH2PO4 1000 μmol/L, P) conditions. The results showed that the light energy conversion efficiency and electron transport rate (ETR) of Qi319-96 were higher than that of Qi319. The light absorbed by maize that entered into the photochemical reaction in Qi319-96 was higher than that in Qi319, whereas thermal energy dissipation through antenna pigments was more weakened. The surplus energy that dissipated by ROS-scavenging system had no significant diversity between Qi319-96 and Qi319 under phosphorus starvation. Inorganic phosphorus content was higher in Qi319-96 in comparison with Qi319 under low phosphorus stress. Compared to Qi319, Qi319-96 possessed higher efficiency of light energy conversion and carbon assimilation. These results indicated that different inorganic phosphorus concentration of leaf may be the major cause for the variation of photosynthetic characteristics between Qi319-96 and Qi319.

References

[1] Nilsson L, Müller R, Nielsen T H. Dissecting the plant transcriptome and the regulatory responses to phosphate deprivation [J]. Physiologia Plantarum,2010,139:129-143.
[2] Plaxton W C, Tran H T. Metabolic adaptations of phosphate-starved plants[J]. Plant Physiology,2011,156:1006-1015.
[3] Usuda H, Shimogawara K. Phosphate deficiency in maize. IV. Changes in amounts of sucrose phosphate synthase during the course of phosphate deprivation[J]. Plant and cell physiology,1993, 34:767-770.
[4] Usuda H, Shimogawara K. Phosphate deficiency in maize III. Changes in enzyme activities during the course of phosphate deprivation[J]. Plant Physiology,1992,99:1680-1685.
[5] Usuda H, Shimogawara K. Phosphate deficiency in maize. II. Enzyme activities[J]. Plant and cell physiology,1991 32: 1313-1317.
[6] Sharkey T D, Stitt M, Heineke D, et al. Limitation of photosynthesis by carbon metabolism II. O2- insensitive CO2 uptake results from limitation of triose phosphate utilization[J]. Plant Physiology,1986,81: 1123-1129.
[7] Wu P, Ma L, Hou X, et al. Phosphate starvation triggers distinct alterations of genome expression in Arabidopsis roots and leaves[J]. Plant Physiology,2003,132: 1260-1271.
[8] Müller R, Morant M, Jarmer H, et al. Genome-wide analysis of the Arabidopsis leaf transcriptome reveals interaction of phosphate and sugar metabolism[J]. Plant Physiology,2007,143: 156-171.
[9] Morcuende R, Bari R, Gibon Y, et al. Genome-wide reprogramming of metabolism and regulatory networks of Arabidopsis in response to phosphorus[J]. Plant, Cell & Environment,2007, 30: 85-112.
[10] Alexova R, Millar A H. Proteomics of phosphate use and deprivation in plants[J]. Proteomics,2013,13(3-4):609-623.
[11] Da Silva á E, Gabelman W H. Screening maize inbred lines for tolerance to low-P stress condition[J]. Plant and Soil,1992,146: 181- 187.
[12] Li K, Xu C, Li Z et al. Comparative proteome analyses of phosphorus responses in maize (Zea mays L.) roots of wild- type and a low- P- tolerant mutant reveal root characteristics associated with phosphorus efficiency[J]. Plant Journal,2008,55:927-939.
[13] 李绍长,胡昌浩,龚江,等.低磷胁迫对磷不同利用效率玉米叶绿素荧光参数的影响[J].作物学报,2004,30:365-370.
[14] 张可炜,王贤丽,李坤朋,等.低磷胁迫对耐低磷玉米自交系幼苗光合特性的影响[J].山东大学学报,2007,42:89-94.
[15] Li K, Xu C, Zhang K, et al. Proteomic analysis of roots growth and metabolic changes under phosphorus deficit in maize (Zea mays L.) plants[J]. Proteomics,2007,7:1501-1512.
[16] 张可炜,陶佩琳,刘寒寒,等.低磷胁迫下不同基因型玉米对难溶性磷酸盐的吸收和利用[J].中国农学通报,2013,29:28-35.
[17] 鲍士旦.土壤农化分析[M].北京:中国农业出版社.2000,P76
[18] Taussky H H, Shorr E. A microcolorimetric method for the determination of inorganic phosphorus[J]. Journal of Biological Chemistry,1953,202:675-685.
[19] Aron D I. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris[J]. Plant physiology,1949, 24: 1- 15.
[20] 罗广华,王爱国.植物的超氧物自由基与羟胺反应的定量关系[J].植物生理学通讯,1990, 6: 55-57.
[21] MacNevin W, Urone P. Separation of hydrogen peroxide from organic hydroperoxides[J]. Analytical Chemistry,1953, 25: 1760- 1761.
[22] Patterson B D, MacRae E A, Ferguson I B. Estimation of hydrogen peroxide in plant extracts using titanium (IV) [J]. Analytical Biochemistry,1984,139:487-492.
[23] Giannopolitis C N, Ries S K. Superoxide dismutases: I. Occurrence in higher plants[J]. Plant Physiology,1977,59:309.
[24] Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbatespecific peroxidase in spinach chloroplasts[J]. Plant and cell physiology,1981,22:867-880.
[25] Pei L, Wang J, Li K, et al. Overexpression of Thellungiella halophila H +- pyrophosphatase gene improves low phosphate tolerance in maize[J]. PloS one,2012,7:e43501.
[26] Demmig- Adams B, Adams III WW, Barker D H, et al. Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation[J]. Physiologia Plantarum,1996,98:253-264.
[27] 樊广华,谷淑波,宁堂原,等.生物发光法测定玉米籽粒中 ATP的含量[J].现代仪器,2005,3:34-35.
[28] Farquhar G D, Sharkey T D. Stomatal conductance and photosynthesis[J]. Annual review of plant physiology,1982,33:317- 345.
[29] 徐建旭,周慧芬,邱翠花,等.高温强光下温州蜜柑光合机构运转与叶黄素循环和 D1蛋白周转的关系[J].园艺学报,2011,38:205-214.
[30] Pérez P, Rabnecz G, Laufer Z, et al. Restoration of photosystem II photochemistry and carbon assimilation and related changes in chlorophyll and protein contents during the rehydration of desiccated Xerophyta scabrida leaves[J]. Journal of Experimental Botany,2011, 62: 895-905.
[31] 李惠华,赖钟雄. 植物抗坏血酸过氧化物酶研究进展[J].亚热带植物科学,2006,35:66-69.
[32] Calderón-Vázquez C, Alatorre-Cobos F, Simpson-Williamson J, et al. Maize under phosphate limitation. Handbook of Maize: Its Biology[M]. Springer.2009:381-404.
[33] Fredeen A L, Rao I M, Terry N. Influence of phosphorus nutrition on growth and carbon partitioning in Glycine max[J]. Plant Physiology1989,89:225-230.
[34] Li X N, Ashihara H. Effects of inorganic phosphate on sugar catabolism by suspension- cultured Catharanthus roseus[J]. Phytochemistry,1990,29:497-500.
[35] Jacob J, Lawlor D. In vivo photosynthetic electron transport does not limit photosynthetic capacity in phosphate- deficient sunflower and maize leaves[J]. Plant, Cell & Environment,1993,16:785-795.
[36] Asada K. The water–water cycle as alternative photon and electron sinks[J]. Philosophical Transactions of the Royal Society of London Series B: Biological Sciences,2000,355:1419-1431.
[37] Asada K. Production and scavenging of reactive oxygen species in chloroplasts and their functions[J]. Plant Physiology,2006,141:391- 396.
[38] Hu X, Jiang M, Zhang A, et al. Abscisic acid- induced apoplastic H2O2 accumulation up- regulates the activities of chloroplastic and cytosolic antioxidant enzymes in maize leaves[J]. Planta,2005,223: 57-68.
[39] Fredeen A L, Raab T K, Rao I M, et al. Effects of phosphorus nutrition on photosynthesis in Glycine max (L.) Merr[J]. Planta, 1990,181:399-405.
[40] Jacob J, Lawlor D W. Dependence of photosynthesis of sunflower and maize leaves on phosphate supply, ribulose- 1,5- bisphosphate carboxylase/oxygenase activity, and ribulose-1, 5-bisphosphate pool size[J]. Plant Physiology,1992,98:801-807.