Abstract: It’s hard to sell grapes if they were pushed to market on the same time, for this reason, the changes of weight loss, the percentage of good fruit, soluble sugar content, titratable acid content and ascorbic acid content of‘New Manicure Finger’grapes with different maturities by controlling picking time were studied after the grapes were packed with PE film bags for modified atmosphere cold storage. The results suggested that the 80% maturity performed the best among the four maturities, its weight loss was less than 5%, and the percentage of good fruit accounted for 74% after stored for 50 days. Furthermore, the titratable acid content of 80% maturity reached the greatest value (0.3%), differed significantly with the other three maturities (P＜0.01) after stored for 50 days. However, the soluble sugar content of 80% maturity reached a peak (16.25%) after stored for 40 days. In addition, the ascorbic acid of 80% maturity varied slightly among the whole storage. In conclusion, the best picking time of‘New Manicure Finger’grapes was when they reached grade 8 (80% maturity), which was good for storage, preservation, quality and flavoring.