The Influence of Chinese Herbal Medicine Feed Additiveon on Eel(Monopterus albus) Sex Hormone Levels

doi:10.11924/j.issn.1000-6850.2014-0591

Abstract

Abstract: The female eel of initial average weight (41.2 2.3) g and the male eel of initial weight (113.5 5.6) g were took as the research objects. The test used minced fish feed with concentration of Chinese herbal medicine at the rate of 3%, 4%, 5%, respectively, and set up three repeats for each group. Continuous feeding was conducted for 31d (the first 10d was the training phase without Chinese herbal medicine, followed by 21d of trial phase with Chinese herbal medicine added). Blood sample was obtained to determine the content of serum estradiol and testosterone and evaluate the influence of the concentration of Chinese herbal medicine on sex hormone levels of eel. The experimental results show that: (1) compared with control group, estradiol and testosterone levels in blood of parent fish that fed without Chinese herbal medicine were significantly higher than that fed with Chinese herbal medicine (P <0.05), and serum levels of estradiol and testosterone decreased with the increase of concentration of Chinese herbal medicine (P <0.05); (2) the serum estradiol level of females and serum testosterone level of males declined with time (P <0.05) in each test group.

References

[1] 谢仲权, 牛淑琦. 天然植物饲料添加剂生产技术与质量标准[M]. 北京: 中国农业科学技术出版社, 2005: 23–28.
[2] 王吉桥, 孙永新, 张剑诚. 金银花等复方草药对牙鲆生长、消化和免疫能力的影响[J]. 水产学报, 2006, 30(1): 90–96.
[3] 邱小琮, 周洪琪, 刘小刚, 等. 中草药添加剂对异育银鲫生长和蛋白质消化吸收的影响[J]. 水产学报, 2002, 26(6): 551–555.
[4] 王永奇, 刘文华,蒋志武, 等花面狸药物催情的初步试验研究[J]. 陕西师范大学学报（自然科学版）, 2003, 31(2): 140-143.
[5] 第一五七医院, 广州部队军区后勤部卫生药检室. 新编中医学[M]. 北京:中国人民解放军战士出版社, 1980: 409-447.
[6] 向枭, 周兴华, 唐龙碧. 中草药添加剂在水产动物营养上的作用[J]. 中国饲料, 2000,21: 23-24.
[7] 唐黎, 王吉桥, 陈玉珍, 等. 复方中草药制剂对瓦氏黄颡鱼生长的影响[J]. 贵州农业科学, 2010, 38(11): 194–195.
[8] 艾国良, 许美解, 曾元根, 等. 中草药对王鸽繁殖性能影响的比较实验[J]. 养禽与禽病防治, 1996: 12-13.
[9] 付永忠, 王云新, 黄国光, 等. 斜带石斑鱼亲鱼强化培育及自然产卵研究[J]. 中山大学学报:自然科学版, 2001, 39(6): 81–85.
[10] 贾斌, 谷新利, 张林辉, 等. 催情促孕散对发情期绵羊子宫和输卵管肌电活动的影响[J]. 中国兽医杂志, 2000, 26(4): 38–39.
[11] 王吉桥, 柳圭泽, 田信忠, 等. 中草药添加剂对黄颡鱼繁殖性能及效果的影响[J]. 大连水产学院学报, 2008, 23(4): 258–262.
[12] 石琼, 孙儒泳. 黄鳝的性转变[J]. 生物学通报. 1999, 34（5）: 12–13.
[13] 陈全震, 高爱根. 黄鳝养殖生物学的研究进展[J]. 水产科技情报. 1999, 26(3): 115–119.
[14] 胡宝林. 黄鳝养殖技术之三—黄鳝人工繁殖及苗种培育技术[J]. 中国水产,2004,（2）：42–44.
[15] 王文彬, 张修协, 曾伯平, 等. 中草药制剂应用于黄鳝人工繁殖的初步试验[J]. 水利渔业, 2007, 27(6): 28–29.
[16] 邴旭文. 模仿自然繁殖条件下的黄鳝人工繁殖试验[J]. 水产学报, 2005, 29(2): 285–288.
[17] 章振保, 杨庆涛, 杨镜秋, 等. 淫羊藿甙、菟丝子提取物对雄激素部分缺乏大鼠生殖保护作用的比较研究[J]. 中国老年学杂志, 2006, （26）: 1389–1391.
[18] Yang M H, Wang W Q, Jin Z H,et al. Study on effects fresh Yimucao contraction uterus[J].Primary J Chin Mater Med(基层中药杂志), 2001, 15(3): 61–62.
[19] Ma Y M, Yang D Y, Tian Z F,et al. Effect of motherwort herb on myoelectric activity of uterus in rats [J].China J Chin Mater Med(中国中药杂志), 2000, 25(6): 364–366.
[20] Chan W C, Wong Y C, Kong Y C,et al. Clinical observation on the uterotonic effect of l-mu Ts' ao (Leonurus artemisia)[J].Am J Chin Med, 1983, 11(1-4): 77–83.
[21] 王若光, 尤昭玲冯光荣. 中药植物性雌激素研究 [J]. 中国中西医结合杂志, 2004, 24(2): 169–171.
[22] 阮金兰, 杜俊蓉, 曾庆忠, 等. 益母草的化学、药理和临床研究进展[J]. 中草药, 2003, 34(11): 15–19.
[23] 赵青威, 楼宜嘉. 黑大豆乙醇提取物的雌激素样作用及其机制研究[J]. 中国中药杂志, 2006, 31(10): 825–828.
[24] 王庆奎, 陈成勋, 郭永军, 等. 7 种中草药对斑点叉尾鮰生理生化指标的影响[J]. 安徽农业科学, 2011, 39(15): 9305–9308.
[25］国家药典委员会．中华人民共和国药典:一部[S]. 北京: 化学工业出版社，2005: 201．
[26] 杨海, 彭风, 胡建宏, 等. 3种中草药方剂对家兔精液及精子品质的影响[J]. 西北农林科技大学学报, 2012, 40(12): 1–5.
[27] Tan Q H, Sun Z W. Effect of vitamin C and Chinese herb additives on the sperm quality of rooster in summer[J]. Journal of Gansu Agricultural University, 2009, 44(4): 36–40.
[28] Long X, Tai X L. Effect of Chinese herbal additives on the quality of boar semen[J] Sichuan Animal Husbandry and Veterinary,1998(3): 23.
[29] Li Y Q, Feng B. Treatment of bulls whose semen was poor quality with Chinese herbal medicine[J]. Shandong Animal Husbandry and Veterinary, 2007, 28(4): 6–7.
[30] Sun Y B, Song X C, Liu X W,et al. Effect of compound Chinese medicine herb additives on productive hormones of Liaoning cashmere ram[J]. Feed Industry, 2012, 33(7): 45–47.
[31] Sun Y B, Song X C, Liu X W,et al Effect of compound Chinese medicine herb additives on semen character of Liaoning cashmere rams[J]. Modern Animal Husbandry and Veterinary, 2012(2): 34–37.
[32] 宋平, 熊全沫. 黄鳝血清睾酮和雌二醇含量周年变化规律的研究[J]. 武汉大学学报. 1993, （5）: 115–120.
[33] 王吉桥, 柳圭泽, 阎彦春, 等. 在饲料中添加中草药对黄颡鱼性腺发育的影响[J]. 北京水产, 2008, (4): 27–34.