Research Methods and Progress on Molecular Ecology of Compost Microorganism

doi:10.11924/j.issn.1000-6850.2014-0761

Abstract

Abstract: Composting is one of the effective methods of disposing organic solid wastes. The microorganisms play significant roles in the composting process. Therefore, it is of great importance for researchers to accelerate fast maturity by studying microbial communities and dynamic changes during composting process. The traditional methods (cultivation approach, direct microscopic count and specific functional microbial study technique) and modern molecular biology technologies (modern biochemical method, modern molecular biology technology, high-throughput sequencing and other molecular biology methods) of studying compost microorganism were reviewed in this paper, and the advantages and drawbacks of traditional methods and modern molecular biology technologies to research the compost microbial were also deeply analyzed. Finally, the proper choice of research methods of studying compost microbial structure and its dynamic change were proposed.

Wang Zhenchuan,Sun Gaige,Liu Qiang,Xie Jun and Xie Guixian. Research Methods and Progress on Molecular Ecology of Compost Microorganism[J]. Chinese Agricultural Science Bulletin, 2014, 30(33): 110-115.

References

[1] Zhu N, Deng C, Xiong Y, et al. Performance characteristics of three aeration systems in the swine manure composting [J]. Bioresource Technology, 2004, 95(3):319-326.
[2] 李国学,张福锁.固体废物堆肥化与有机复混肥生产[M].北京:化学工业出版社,2000.
[3] Amann R I, Ludwig W, Schleifer K H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation[J]. Microbiological Reaearch, 1995, 59(1):143-169.
[4] 张高娜,张建梅,谷巍.微生物分子生态学研究进展[J].微生态新技术,2013(20)50-51.
[5] 易境.猪场废弃物堆肥中芽孢杆菌属和梭菌属细菌的分子生态学研究[D].武汉:华中农业大学,2013.
[6] 张嘉超.基于分子生物学的堆肥微生物种群与体系基质特性关系研究[D].长沙:湖南大学,2013.
[7] 焦晓丹,吴凤芝.土壤微生物多样性研究方法的进展[J].土壤通报,2004,35(6):790-791.
[8] 朱海霞,陈林海,张大伟,等.活性污泥微生物菌群研究方法进展[J].生态学报,2007,27(1):315-321.
[9] 冯明谦,刘德明.滚筒式高温堆肥中微生物种类数量的研究[J].中国环境科学,1999,19(6):1-5.
[10] Boha S V, Mihelic R, Lobnik F, et a1. Microbial community structure during composting with and without massinocula[J].Compost Science and Utilization,2003,11(1):6-15.
[11] 叶劲松,吴先,蔡敬民,等.好氧堆肥微生物研究方法进展[J].安徽农业科学,2008, 36(30): 13287-13291.
[12] 王晓芳,徐旭士,吴敏,等.一株纤维索分解菌的分离与筛选[J].生物技术,2001,11(2):28-29.
[13] 叶姜瑜.一种纤维素分解鉴别培养基[J].微生物通报,1997,24(4):251-2521.
[14] 张洪勋,王晓谊,齐鸿雁.微生物生态学研究方法进展[J].生态学报,2003, 23(5):988- 995.
[15] Yu H Y, Zeng G M, Huang H L, et al. Microbial community succession and lignocellulose degradation during agricultural waste composting [J].Biodegradation,2007,18(6):793-802.
[16] 张建栋,王梦亮,刘滇生.PLFA方法对堆肥化过程中微生物群落结构变化的影响[J].安徽农业科学,2006,34(3):534-535.
[17] 张园,耿春女,何承文,等.堆肥过程中有机质和微生物群落的动态变化[J].生态环境学报,2011,20(11):1745-1752.
[18] 胡洪营,童中华.微生物醌指纹法在环境微生物群体组成研究中的应用[J].微生物学通报, 2002,29(4): 95- 98.
[19] 唐景春,Katayama A. 醌类图谱分析在环境微生物生态测定中的应用[J].应用与环境生物学报,2004,10(4):530-536.
[20] Hiraishi A, Yamanaka Y, Narihiro T. Seasonal microbial community dynamics in a flowerpot-using personal composting system for disposal of household biowaste[J].The Journal General Applied Microbiology, 2000,46(3):133-146.
[21] Peter M D, William C G. Microbial diversity in hot synthetic compost as revealed by PCR amplified rRNA sequences from cultivated isolates and extracted DNA[J]. FEMS Microbiology Ecology, 2001,35 (2):207-216.
[22] LanMontagn M G, Michel J F C, Holden P A, et al. Evaluation of extraction and purification methods for obtaining PCR amplifiable DNA from compost for microbial community analysis[J] . Journal of Microbiological Methods, 2002,49(3):255-264.
[23] 曾光明,黄国和.堆肥环境生物与控制[M].北京:科学出版社,2006.
[24] Pasi P, Jenni H, Lars P, et al. Bacterial diversity at different stages of the composting process [J]. BMC Microbiology, 2010, 10(1):94.
[25] 赵冬梅,顾文杰,张发宝,等.1株好氧堆肥高温H2S脱臭菌的筛选、鉴定及生长特性[J].微生物学杂志,2012,32(6):47-51.
[26] 徐庆贤,官雪芳,林碧芬,等.几株猪粪堆肥发酵菌对堆肥发酵的促进作用[J].生态与农村环境学报,2013,29(2):253-259.
[27] 王丽芳,陈婷婷,王琪,等.芦笋老茎堆肥中嗜热细菌的分离与鉴定[J].微生物学杂志,2013,33(1):8-13.
[28] 张蔓,魏明宝,马闯,等.分子生物学技术在堆肥微生物研究中的应用综述[J].郑州轻工业学院学报:自然科学版,2012,28(3):13-17.
[29] 邵冠军,吕杰,马媛.分子生态学技术应用于新疆环境微生物研究进展[J].安徽农业科学,,2013, 41(6):2394-2398.
[30] 李琬,李景鹏.分子生物学技术在堆肥微生态研究中的应用研究进展[J].中国农学通报,2012,28(18):20-25.
[31] Ishii K, Fukui M, Takii S, et a1. Microbial succession during a composting process as evaluated by denaturing gradient gel electrophoresis analysis[J].Journal of Applied Microbiology,2000,89(5):768-777.
[32] 王伟东,王小芬,朴哲,等.堆肥化过程中微生物群落的动态[J].环境科学,2007,28(11):2593-2597.
[33] 刘佳,许修宏,李洪涛.利用PCR-DGGE技术对自然堆肥微生物群落多样性的分析[J].东北农业大学学报,2009,40(9) :35-38.
[34] 虞泳,曾光明,陈耀宁,等.农业废物好氧堆肥中氨氧化细菌的群落结构[J].环境科学,2011,32(10) :3068-3071.
[35] Head I M, Saunders J R, Pickup R W. Microbial evolution, diversity,and ecology: A decade of ribosomal RNA analysis of uncultivated microorganisms[J]. Microbial Ecology, 1998, 35(1):1-21.
[36] Alfreider A, Peters S, Tebbe C C, et al. Microbial community dynamics during composting of organic matter as determined by 16S Ribosomal DNA Analysis[J]. Applied Microbiology and Biotechnology,2006,71(6) :942-952.
[37] Sabine P, Stefanie K, Frank S, et al . Succession of microbial communities during hot composting as detected by PCR-Single-Strand-Conformation Polymorphism-Based genetic profiles of small-subunit rRNA genes[J]. Applied and Environment Microbiology, 2000,66(3):930-936.
[38] 王兴春,杨致荣,王敏,等.高通量测序技术及其应用[J].中国生物工程杂志,2012,32(1):109-114.
[39] 王绍祥,杨洲祥,孙真,等.高通量测序技术在水环境微生物群落多样性中的应用[J].化学通报,2014,77(3):196-201.
[40] 闫绍鹏,杨瑞华,冷淑娇,等.高通量测序技术及其在农业科学研究中的应用[J].中国农学通报,2012,28(30):171-176.
[41] 秦楠,栗东芳,杨瑞馥,等.高通量测序技术及其在微生物学研究中的应用[J].微生物学报,2011,51(4):445-457.
[42] 贾洋洋.利用宏基因组方法分析堆肥生境中微生物区系的变化[D].泰安:山东农业大学,2012.
[43] 喻曼,许育新,曾光明,等.RFLP法研究接种对农业废物堆肥微生物多样性的影响[J].农业环境科学学报,2010,29(2):396.
[44] 徐哓飞,林炜铁,彭智辉,等.RAPD技术及其在微生物学方面的应用[J].生命的化学,2001(5):436.