Identification and Pathotype Analysis of Pepper Mild Mottle Virus in Processing Pepper from Xinjiang

doi:10.11924/j.issn.1000-6850.2014-0836

Abstract

Abstract: To identify the pathotype of PMMoV infecting processing pepper in Xinjiang, some disease samples collected from southern Bavaria were detected by double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) and identified with reverse transcription-polymerase chain reaction (RT-PCR), cloning and sequence analysis. The results showed that PMMoV was detected from pepper plants, pepper fruit and seed samples. Three target amplified fragments with sizes of 576 bp were cloned, sequenced and analyzed. The results indicated that the similarity of the nucleotide and deduced amino acid sequences of CP gene showed highly identity between PMMoV–XJ isolates and that have been reported, sharing of 89.0%- 99.2% and 95.5%- 100.0% sequences identity respectively, and was most closely related to PMMoV- Israel strain EF434393. High sequence identities of CP gene were showed among Xinjiang isolates. Phylogenetic analysis based on the sequences of CP gene, the PMMoV isolates in Xinjiang processing pepper were belonged to P_1,2 pathotype.

References

[1] CAB International. Crop Protection Compendium (2005 Edition) [DB].Wallingford.UK: CAB International,2005, www. abicompendium.org/CPc.
[2] 向本春,谢浩,崔星明,等.新疆辣椒轻微斑驳病毒的分离鉴定[J].病毒学报,1994,10(3):240-245.
[3] 黄粤,马荣群,翟晓灵,等.辣椒温和斑点病毒(PMMoV)外壳蛋白基因的克隆和序列测定[J].植物生理学通讯,2005,41(2):205-207.
[4] Wang X, Liu F, Zhou G, et al. Detection and molecular characterization of pepper mild mottle virus in China[J]. J Phytopathology,2006,154:755-757.
[5] 夏惠娟,李志勇,郭京泽,等.保定地区一种新辣椒病毒病的鉴定[J].河北农业大学学报,2006,29(6):65-67,86.
[6] 李兴红,严红,郭京泽,等.种传辣椒轻斑驳病毒病 DAS-ELISA的检测[J].植物保护,2005,31(3):66-68.
[7] Colson P, Richet H, Desnues C, et al. Pepper mild mottle virus, a plant virus associated with specific immune responses, Fever, abdominal pains, and pruritus in humans[J]. PloS one,2010,5(4): e10041.
[8] Ikegashira Y, Ohki T, Ichiki U T, et al. An immunological system for the detection of pepper mild mottle virus in soil from green pepper fields[J]. Plant Disease,2004,88(6):650-656.
[9] Matsunaga H, Saito T, Hirai M, et al. DNA markers linked to pepper mild mottle virus(PMMoV)resistant locus(L4)in Capsicum [J]. J Horti Sci Japanese Society,2003,72(3):218-220.
[10] Kang B C, Yeam I, Jahn M M. Genetics of plant virus resistance[J]. Annu. Rev. Phytopathol,2005,43:581-621.
[11] Genda Y, Kanda A, Hamada H, et al. Two amino acid substitutions in the coat protein of pepper mild mottle virus are responsible for overcoming the L4 gene- mediated resistance in Capsicum spp[J]. Phytopathology,2007,97:787-793.
[12] García- Luque I, Serra M T, Alonso E, et al. Characterization of a spanish strain of pepper mild mottle virus (PMMoV- S) and its relationship to other tobamoviruses[J]. J Phytopathol,1990,129:1-8.
[13] Velasco L, Janssen D, Ruiz-Garcia L, et al. The complete nucleotide sequence and development of a differential detection assay for a pepper mild mottle virus(PMMoV)isolate that overcomes L3 resistance in pepper[J]. J Viro Meth,2002,106(1):135-140.
[14] Antignus Y, Lachman O, Pearlsman M, et al. A new pathotype of pepper mild mottle virus(PMMoV) overc- omes the L4 resistance genotype of pepper cultivars[J]. Plant Dis,2008,92:1033-1037.
[15] 杨玲,张荣意,张振臣.植物病毒种群结构及分子变异机制研究进展[J].河南农业科学,2009,2:20-24.
[16] Kazinczi G, Horvath J, Gaborjanyi R. Some aspects of pepper virus research[J]. Acta Phyto. Ento., 2001,36:329-347.
[17] Mijatovic M, Ivanovic M, Obradovic A. Potato virus Y (PVY) on pepper in Serbia[J]. Acta Horticulturae, 2002,579:545-549.
[18] 谭根堂,史联联,尚惠兰,等.陕西线辣椒病毒病病原检测简报[J].辣椒杂志,2003,3:32-33.
[19] 李明福,张振铎,陈燕芳,等.引种辣椒上一种新病害的病原鉴定[J].云南农业大学学报,2003,18(4):96.
[20] Berzal-Herranz A, de la Cruz A, Tenllado F, et al. The Capsicum L3 gene- mediated resistance against the tobamoviruses is elicited by the coat protein[J]. Virology,1995,209:498-505.
[21] Gilardi P, García- Luque I, Serra M T. The coat protein of tobamovirus acts as elicitor of both L2 and L4 gene- mediated resistance in Capsicum[J].J Gen Virol,2004,85:2077-2085.
[22] 廖富荣,沈建国,吴媛,等.台湾甜椒种子中辣椒轻斑驳病毒检测及其致病型鉴定[J].热带作物学报,2011,32(6):1128-1135.
[23] Velasco L, Janssen D, Ruiz-Garcia L, et al. The complete nucleotide sequence and development of a differential detection assay for a pepper mild mottle virus(PMMoV)isolate that overcomes L3 resistance in pepper[J]. J Virol Methods,2002,106:135-140.