Abstract: The bacteria from the suspected haemophilus parasuis infection cases from some boar farms in Fujian Province were isolated and identified, and 16S rRNA of the strains was amplified and sequenced. These strains were identified based on bacterial morphology, PCR identification and sequence analysis. The results showed the sequences homology of the 6 isolations was up to 99%. However, the sequences homology of the isolates with reference strains was ranged from 84.5% to 99.2% . The phylogenetic tree revealed that the genetic relationship of the isolated strains was closer to serovar4 and serovar5, and farther to other serovars. The research developed a technology of isolating and culturing of haemophilus parasuis, and the isolated strains were identified by PCR method and 16S rRNA gene of the isolated strains were sequenced and analyzed. In a word, the research laid a foundation for diagnosis and prevention and cure of the haemophilus parasuis disease.