Abstract: To understand general growth property of Bipolaris oryzae from Leersia hexandra and provide references for disease control, total 6 isolates were assessed for cultural and biological properties. The experiment results indicated that the mycelium could grow at the wide temperature and pH range of 20-35℃ and pH 5-10 under dark or light conditions. The optimal growth condition for these isolates was 25-30℃, pH 7-8 in the light. The mycelium could utilize various sugar sources. Among 5 sugar sources tested, cultures of B. oryzae in the medium containing glucose and maltose resulted in faster hyphal extension rates. Among nitrogen sources tested, these isolates could grow on the NH₄-N, NO₃-N (nitrate) and mixture nitrogen medium, and NO₃-N (nitrate) was advantageous to hyphal extension. However, urea could completely inhibit mycelia growth.