Abstract: The experiment was conducted to learn the effect of different proportion of soybean oil (n-6 PUFAs) and linseed oil (n-3 PUFAs) on antioxidant capacity of young breeder rooster plasma, kidney and testis. 90 10-week-old breeder roosters were randomly divided into 5 groups, each group had 3 repeats, and 6 roosters in each repeat. Using corn- soybean as meal diets, the ratio of soybean oil and linseed oil were: 2% and 0 (Treatment 1, Control), 1.5% and 0.5% (Treatment 2), 1% and 1% (Treatment 3), 0.5% and 1.5% (Treatment 4) and 0 and 2% (Treatment 5). The results showed that: (1) with the increasing doses of LO, plasma T-SOD, CAT and T-AOC activity was increasing first, then decreasing; compared with the Control, plasma T-SOD, CAT and T-AOC activity of Treatment 3 increased 0.38% (P＞0.05), 12.31%(P＞0.05)and 28.24%（P＜0.05）at day 28; compared with the Control, plasma T-SOD, CAT and T-AOC activity of Treatment 3 increased 5.43% (P＞0.05), 10.63% (P＞0.05) and 13.76% (P＜0.05) at day 56; (2) compared with the Control, T-SOD, CAT and TAOC activity of kidney of Treatment 3 increased 9.26% (P＞0.05), 69.36% (P＜0.05) and 4.91% (P＞0.05); compared with the Control, T- SOD, CAT and T- AOC activity of testis of Treatment 3 increased 18.86% (P＞0.05), 103.95% (P＜0.05) and 20.40% (P＞0.05). Under the experiment condition, dietary supplementation with different vegetable oil influences the plasma and tissue antioxidant activity of young breeder rooster, and enhanced the body’s antioxidant capacity. The body’s antioxidant capacity was the best when the dose of soybean oil and linseed oil were 1% and 1%.