Abstract: Orchard soil acidification is increasingly serious with the over-application of fertilizer in these years. The aluminum in acid soil may destroy the root tips and decrease the elongation of root, therefore inhibit the tree growth. Thus, five kinds of one- year- old apple rootstocks [Malus hupehensis Rehd., M. micromalus Makino, M. baccata Borkh., M. prunifolia (Willd) Borkh. and M. sievesii (Ledeb.) Roemer] were used to study the phosphorus acquisition and utilization characteristics under AlPO₄ stress. The results were as follows: organ biomass and plant dry weight of five rootstocks from high to low were Malus hupehensis Rehd.＞M. micromalus Makino＞M. sievesii (Ledeb.) Roemer＞M. prunifolia (Willd) Borkh.＞M. baccata Borkh. Under AlPO₄ stress, phosphorus acquisition efficiency (PAE) of Malus hupehensis Rehd. was the highest (8.85 mg/plant), then followed M. micromalus Makino (6.93 mg/plant), while the PAE of M. baccata Borkh. was the lowest (2.22 mg/ plant). Organ dry weight and plant dry weight of five rootstocks increased significantly after the induction of low volume phosphorus, whereas significant difference could be seen among the five rootstocks M. baccata Borkh. had the most vigorous growth on biomass and PAE, which was increased by 2.17 and 2.27 fold respectively, and then followed by M. prunifolia (Willd) Borkh. (1.55 and 1.74 fold), while that of Malus hupehensis Rehd. increased the minimum, with the biomass and PAE increased 0.44 and 0.38 fold respectively. However, no significant difference was found on the phosphorus utilization efficiency (PUE) of the five rootstocks. In consequence, the growth of Malus hupehensis Rehd. was the best under AlPO₄ stress, then followed by M. micromalus Makino, while the adaptability of M. baccata Borkh. was the worst. AlPO₄ stress lowered the environment pH of the rootstocks compared to adding phosphorus treatment, and the pH declined of Malus hupehensis Rehd. was 1.52, then followed by M. micromalus Makino (0.59), while the pH of M. baccata Borkh. lowered only 0.22.