Cloning and Bioinformatic Analysis of CBF1 Gene in Amygdalus communis

doi:10.11924/j.issn.1000-6850.2014-1877

Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (34): 16-23.doi: 10.11924/j.issn.1000-6850.2014-1877

Special Issue: 生物技术；园艺

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Cloning and Bioinformatic Analysis of CBF1 Gene in Amygdalus communis

Zhang Liang¹, Zhang Zhijun², Li Jiang³, Dai Peihong¹, Tian Jia³, Luo Shuping¹

(¹College of Agronomy, Xinjiang Agricultural University, Urumqi 830052;
²The Nurturing Station for the State Key Laboratory of Subtropical Silviculture,
Zhejiang A&F University, Lin’an Zhejiang 311300;
³College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052)

Received:2014-07-07 Revised:2014-11-23 Accepted:2014-09-30 Online:2015-03-10 Published:2015-03-10

Abstract

Abstract: The AcCBF1 gene was cloned and analyzed in order to identify the role of CBF transcription factors in plant resistance of Amygdalus communis. The CBF1 homologue gene named YZ- AcCBF1 (Genbank Accession No. KJ818900) was isolated from‘Yingzui’(Amygdalus communis) with the technology and method of PCR. The bioinformatics analysis showed that the open reading frame was 729 bp in full- length and encodedaprotein of 242 amino acids. The molecular formula was C1195H1886N346O368S13, and its relative molecular mass was approximately 27.405 kDa and isoelectric point was 7.69. This protein was an instable protein whose deduced half-life was 30 h. Its fat factor was 63.72 and the average of hydrophobic was -0.646. Subcellular localization analysis showed that AcCBF1 protein was localized in the nucleus, it was not a secreted protein but the hydrophilic protein and the transmembrane domain did not exist. Phylogenetic analysis revealed that AcCBF1 encode protein showed the highest similarity with Prunus persica (AGS13699). The prediction of secondary structure of protein indicated that the encoded protein mainly contain 26.86% ofα- helices and 58.68% of the random coil. Three- dimensional structure predicted that AcCBF1 protein confirmed the characteristics of AP2 domain model.

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Cloning and Bioinformatic Analysis of CBF1 Gene in Amygdalus communis

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