Frost Hardiness of Chinese Jujube Shoots Assessed by Electrical Impedance Spectroscopy and Electrolyte Leakage Methods

doi:10.11924/j.issn.1000-6850.2014-1905

Abstract

Abstract: Low temperature mainly limits the growth and productivity of Chinese jujube in winter and spring. The most effective way to avoid these damages in areas subjected to these climatic conditions is to select the least susceptible varieties, so the choice of the right method to determine cold hardiness is extremely important. The one-year-old shoots of seven Chinese jujube cultivars were used to measure the frost hardiness by means of electrical impedance spectroscopy (EIS) and conventional electrolyte leakage (EL) methods after exposed to controlled freezing tests. The changes of EIS parameters (specific resistances r and r₁ in Single-DCE model, specific extracellular resistance r_e, specific intracellular resistance r_i, relaxation time τ and distribution coefficient of relaxation time ψ) and the correlations between the frost hardiness results of two methods were compared in order to find the optimum EIS parameters for measuring the frost hardiness of Chinese jujube. The results showed that after controlled freezing tests, the EIS parameters decreased with the reduced temperature. The frost hardiness estimated by EIS parameters r_e and τ correlated significantly with the frost hardiness assessed by EL method (R=0.840 and 0.879, respectively). The results suggested that the parameters r_e and τ could be applied for measuring frost hardiness of the samples of Chinese jujube after exposed to controlled freezing tests. The EIS method was found to be more superior than the EL owing to its time saving, non-destructive and no need of incubation.

Zhang Zhendong,Gong Ruijuan,Wang Zhe,Gao Jiao,Li Yingyue,Zhang Gang and Pang Xiaoming. Frost Hardiness of Chinese Jujube Shoots Assessed by Electrical Impedance Spectroscopy and Electrolyte Leakage Methods[J]. Chinese Agricultural Science Bulletin, 2015, 31(4): 127-134.

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