Abstract: In order to deeply understand the physiological and biochemical characteristics of wild tea germplasm resources in Guangdong and effectively use wild tea germplasm resources for breeding black tea, eighteen amino acid components, eight soluble sugars, six kinds of catechin components in 14 black tea samples were detected with high performance liquid chromatography (HPLC) technology, and combined with sensory quality evaluation, to screen black tea germplasm in Luokeng tea group. The HPLC results showed that EGC had an absolute superiority in catechin, between 15-60 mg/g, and LX4-1, LX4-2 and JG5-1 had higher contents of the total six catechins, they were 118.8, 88.2 and 106.2 mg/g. Maltose was the main component in soluble sugars, between 7-18 mg/g, NJW6-2, SK1-2 and LX4-2 had higher contents of the total eight soluble sugar components, they were 64.1, 40.1, 41.0 mg/g; theanine was the main component in the amino acids, between 10.3-23.0 mg/g, and LX4-1, DBT9-1 and FSP7-2 had higher contents of the total 18 amino acids, they were 42.0, 41.2, 42.5 mg/g. The sensory quality evaluation results showed that HJY3-1 and LX4-1 were rich in almond fragrance, JG5- 1 had special bitterness, NJW6- 2 had very strong sweetness. The results showed that different wild black tea had high diversity in biochemical component content，and HJY3-1, LX4-1, JG5-1, NJW6-2 were excellent germplasms to black tea breeding.