Abstract: In order to reduce white pollution and the labor cost of clearing the mulch in flue-cured tobacco mulching cultivation, effect of biodegradable mulch on yield and quality of flue-cured tobacco as well as the degradation process of biodegradable mulch in field were investigated in this study by utilizing the field randomized block experiment. As the results showed that film mulching, which could promote fast recovery of survival of tobacco seedlings, was conducive to enhance and improve the yield and quality of flue-cured tobacco. Both biodegradable mulch and ordinary plastic could shorten the survival period of flue-cured tobacco seedling, while there was no significant difference in heat preservation and increasing temperature to the field ridges. Furthermore, the upper and middle class tobacco leave proportion of biodegradable mulch treatment was 5.82 percentage points higher than that of none mulching, with yield increase of 32.85 kg/hm² and output value increase of 813.75 yuan/hm². Compared with ordinary film mulch, the yield and the upper and middle class tobacco leave proportion of biodegradable mulch treatment were slightly lower but had no significant difference, with slightly insufficient leaf thickness and color. Finally, the biodegradable mulch film began to crack in the rosette stage, and completely degraded before the field was ploughed. So it is possible for the application of biodegradable mulch in flue-cured tobacco mulching cultivation, but the cracking time is not synchronous to flue-cured tobacco growth period, and the degradability should be improved.