Abstract: In order to identify introduced beet varieties and analyze the genetic relationship between different beet varieties, fingerprinting and genetic relationship analysis of 32 introduced beet varieties were made by SSR primers. 21 pairs of primers with distinct band and easy to identify were screened out of 101 pairs of SSR primers. From the 21 pairs of primers, a total of 127 allelic sites were detected, of which polymorphic site came to 107, with the polymorphism rate reaching 83.6%. The allelic site amplified from each pair of SSR primer amounted to 2-11. On average, each pair of primer amplified 6.1 genotypes, and the amplified stripe size ranged from 90 to 500 bp. The fingerprinting of 32 varieties was constructed by 3 pairs of primers of SB04, S6 and S7. UPGMA cluster analysis of genetic distance showed that all of the varieties were divided into three classes at the genetic distance of 0.17. It indicated that the genetic basis of introduced varieties was narrow. The cluster analysis result well accorded with the origin of the beet variety, which showed that the beet varieties which gathered together basically came from the same company or country.