Abstract: The study on spatial distribution of taxol in plant could provide a scientific basis for reasonable development of Taxus chinensis plant resource. This study investigated the paclitaxel content in leaves, barks and branches of different periods and the taxol content in the leaves under light condition in a plant of Taxus chinensis var. mairei, with high performance liquid chromatography (HPLC). The results showed that the taxol content in the bark (11-17 mg/kg) gradually increased, taxol content in branches (13-18 mg/kg) and leaves (4-14 mg/kg) decreased with accumulated time. The taxol content in the bark of the new branches (18 mg/kg) and in new needles (14 mg/kg) was higher than that in old ones. And taxol content in the leaves under light condition (26 mg/kg) was significantly higher than that in dark condition (2 mg/kg). For industrial extraction of taxol, it suggests that new branches and leaves which are renewable and have high biomass could be used to get more products of taxol.