Abstract: The effects of different nursery ways, such as nursery pot, nursery tray and nutrition substrate block, on the growth of bitter gourd seedlings were studied to select the best way of bitter gourd seedling. The growth index, including speed of seedling, stem diameter, internode length, leaves number, leaves length, leaves width, ground dry weight, ground fresh weight, dry weight and fresh weight of roots of bitter gourd seedlings grown in different seedling cultivation ways were investigated in order to analyze seedling indexes. The nursery pot seedling was the best way in three seedling cultivation ways, the stem diameter of seedlings reached the maximum level of 3.02 mm; the maximum levels of leaf length and width were 5.77 mm and 6.23 mm, respectively; the fresh weight and dry weight of roots were 1.45 g and 0.08 g, respectively; the ground fresh weight and ground dry weight were 4.14 g and 0.35 g, respectively; the seedling index was 0.18. The seedlings of bitter gourd grown in the nursery pots were stronger with a thicker stem, a wider leaf, more roots and moderate height, and the seedling index was the largest. Consequently, nursery pots should be the best method to cultivate the strong seedlings for bitter gourd.