Abstract: The core primers were screened for identification of beet hybrid. The materials were collected from the imported sugarbeet varieties which planted in 16 larger areas in our country, for screening published 130 SSR primers and 70 pairs of beet EST-SSR. Finally, 24 beet primers amplification bands which were clear and had good repeatability and core of high polymorphism were selected from 200 pairs of primers, with 22 PIC of primers value of 24 primers were greater than 0.5, there were seven pairs of primers value were greater than 0.8 among them, and they could be used for sugarbeet variety of first choice for fingerprint build core primers. Cluster analysis was carried out for 16 beet varieties with these core primers, the results of 16 varieties were divided into three classes, and varieties from the same company gathered together basically. Screening of sugar beet SSR core primer would accelerate the identification speed of beet variety purity and authenticity, and would also accelerate the development of molecular biology and other fields of sugar beet.