Cloning and Expression of Cytoplasmic Male Sterility Associated Genes in Sugar Beet and Its Relationship to CMS

doi:10.11924/j.issn.1000-6850.casb14120099

Abstract

Abstract: Previous studies indicated that Mitochondrial atpA gene and orf187 fragment were closely related to male sterility in Crops. In order to research the relationship of atpA gene with orf187 fragment and cytoplasmic male sterility in sugarbeet, atpA gene and orf187 fragment in sugarbeet were isolated and cloned using homology- based technique. The sequence differences in sugarbeet sterile line and its corresponding maintainer line were defined according to sequence analysis, expression analysis of atpA gene and orf187 fragment was studied by further use of the semi- quantitative RT- PCR and Real- time PCR. The results indicated that compared with maintainer line, sterile line atpA had the replacement of bases and insertion; 6 base pairs were missing in sterile line orf187. The expression analysis demonstrated that expression of atpA in sugarbeet sterile line was significantly lower than that in maintainer line besides the big flower bud period, while expression of orf187 in sugarbeet sterile line was apparently higher than that in maintainer line besides the big flower bud period. These results indicated that structural variation and abnormal expression pattern of atpA and orf187 has a close relationship with sugarbeet cytoplasmic male sterility.

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