Investigation and Analysis of the Subtype of Porcine Endogenous Retrovirus in Breeding Base of Meishan Pigs in Shanghai

doi:10.11924/j.issn.1000-6850.casb15010020

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (14): 39-43.doi: 10.11924/j.issn.1000-6850.casb15010020

Special Issue: 生物技术；畜牧兽医

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Investigation and Analysis of the Subtype of Porcine Endogenous Retrovirus in Breeding Base of Meishan Pigs in Shanghai

Wu Yunlong^1,2,3, Zhang Defu^2,3, Wu Caifeng^2,3, Zhang Shushan^2,3, Wang Xin^1,2,3, Dai Jianjun^2,3

(¹College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306;²Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106; ³Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-genetics and Breeding, Shanghai 201106)

Received:2015-01-05 Revised:2015-03-05 Accepted:2015-03-16 Online:2015-06-02 Published:2015-06-02

Abstract

Abstract: In order to provide actual evidence of biological safety for using Meishan pigs for donor organ transplantation, existence and subtype of porcine endogenous retrovirus (PERV) in Meishan pigs were investigated and analyzed respectively. Tissues of 45 pig ears were collected randomly from Meishan pig breeding center in Jiading District, Shanghai. Ear fibroblast cell lines from these tissues were established. The existence of core protein gene (gag), polymerase gene (pol) and capsule membrane gene (env) from PERV provirus in genomic DNA was detected by PCR and expression of mRNA of each gene was tested by RT-PCR. The results showed that three subtypes PERV provirus existed in all 45 DNA samples. Results of RT-PCR showed that PERV-A and PERV-B both expressed in 40 samples, subtype PERV-B expressed only in 5 samples, and subtype PERV-C was not found in this study. In consideration of biological safety, whether these pigs can be used as human xenotransplantation donors still need further discussion.

Wu Yunlong,Zhang Defu,Wu Caifeng,Zhang Shushan,Wang Xin and Dai Jianjun. Investigation and Analysis of the Subtype of Porcine Endogenous Retrovirus in Breeding Base of Meishan Pigs in Shanghai[J]. Chinese Agricultural Science Bulletin, 2015, 31(14): 39-43.

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Investigation and Analysis of the Subtype of Porcine Endogenous Retrovirus in Breeding Base of Meishan Pigs in Shanghai

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