Abstract: Mytilaria laosensis, Pinus massoniana, Cunninghamia lanceolata, Cinnanomum camphora, Castanopsis hystrix and Michelia macclurei were used to study the litter storage, water holding capacity, water holding rate and water absorption rate, in order to understand the ecohydrological characteristics of the six typical native trees in southern China comprehensively. Field sampling and laboratory analysis were conducted to express the litter storage and water holding capacity of these trees quantitatively. The result showed that the litter storage in the six similar aged native trees decreased in order of M. laosensis (2.220×10³ kg/hm²)>M. macclurei (2.105×10³ kg/hm²)>C. camphora (2.051×10³ kg/hm²)>C. hystrix (1.434×10³ kg/hm²)>C. lanceolata(1.225×10³ kg/hm²)>P. massoniana (0.898×10³ kg/hm²). The order of total water holding capacity of litter was M. laosensis>M. macclurei>C. hystrix>C. camphora>C. lanceolata>P. massoniana. The water holding capacity of the litter in M. laosensis, M. macclurei, C. hystrix, C. camphora, C. lanceolata, P. massoniana six native trees was 458%, 403%, 408%, 404%, 351%, 280%, respectively. The water holding capacity and water holding rate of the litter increased with the soaking time, exhibiting a logarithmical relationship, while a power function presented the relationship between the water absorption rate and the soaking time. Our study established the foundation of evaluating the ecohydrological characteristics of the native trees in south China.