Abstract: In order to reduce the empty shell ratio and improve nut quality of Chinese chestnut, to seek suitable growth regulator spraying method, with chestnut ‘Zunyu’ as test material, the author sprayed different growth regulators at different stages of chestnut, and explored the impact of growth regulators on fruiting conditions and nut quality. The results showed that: at the beginning of full bloom, spraying SA could significantly improve the fruiting rate, the number of nuts in each bud, soluble sugars content, starch content and protein content of chestnut, which could be increased by 15.50%, 79.64%, 19.20%, 11.20% and 6.09%, respectively, compared to that of the treatment CK, and the best spraying was 0.2 nmol/L. Spraying 0.01 mg/L BR before the period of immature embryo development could significantly increase the fruiting rate, soluble sugar content and protein content, which could be increased by 14.16%, 26.37% and 5.69%, respectively, and spraying 0.001 mg/L BR before the period of immature embryo development could significantly increase the fruiting rate and the number of nuts in each bud, which could be increased by 19.42% and 125.56%, and the number of nuts in each bud could be increased up to 125.56%, significantly improve the yield of chestnut. Therefore, it is recommended to spray 0.2 nmol/L SA at the beginning of full bloom and spray 0.001 mg/L BR or 0.01 mg/L BR before the period of immature embryo development, to improve the yield and quality of chestnut.