Screening and Identification of Bacterial Biocontrol Agents Controlling Cucumber Powdery Mildew

doi:10.11924/j.issn.1000-6850.casb15010101

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (16): 160-165.doi: 10.11924/j.issn.1000-6850.casb15010101

Special Issue: 园艺

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Screening and Identification of Bacterial Biocontrol Agents Controlling Cucumber Powdery Mildew

Li Weili¹, He Pengfei^1,2, Wu Yixin^2,3, Qi Chao¹, He Yueqiu^1,3

(¹National Engineering Center for Applied Techniques of Agricultural Biodiversity, Yunnan Agricultural University, Kunming 650201;²National and Local Joint Engineering Research Center for Microbial Screening and Application, Kunming 650217; ³Faculty of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201)

Received:2015-01-15 Revised:2015-05-22 Accepted:2015-04-01 Online:2015-07-27 Published:2015-07-27

Abstract

Abstract: In order to utilize bioagent sources to control cucumber powdery mildew, 25 strains from cucumber rhizospheric bacteria and 91 endophytic bacterial strains of tobacco were used to screen antagonistic bioagents by dual culture experiment with the indicator pathogens Fusarium solani, F. graminearum and Colletotrichum gloeosporioides. The bioagents controlling cucumber powdery mildew were searched from the antagonistic bioagents through pot culture test. The effective bioagents were identified based on colony phenotype, physiological and biochemical characteristics and gyrB gene sequence by PCR. The results showed that 18 bioagents were screened from the 116 bacterial strains and could inhibit the indicators effectively. The endophytic YN-201490 and rhizospheric WL-1 could control the disease with efficiency of 64.86% and 61.26% in the pot culture test when they were sprayed three times with 1.81×10⁸ cfu/mL. YN-201490 and WL-1 were identified as Bacillus subtilis and B. amyloliquefaciens, respectively.

Li Weili,He Pengfei,Wu Yixin,Qi Chao and He Yueqiu. Screening and Identification of Bacterial Biocontrol Agents Controlling Cucumber Powdery Mildew[J]. Chinese Agricultural Science Bulletin, 2015, 31(16): 160-165.

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Screening and Identification of Bacterial Biocontrol Agents Controlling Cucumber Powdery Mildew

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