Abstract: In order to accurately detect wheat pollen activity, five conventional wheat varieties and four candidate liquid medium were chosen as the study materials, and the experiment was conducted to select a more excellent and suitable wheat pollen germination system in vitro culture. Results showed that the germination rate of wheat mature pollen was induced up to more than 90% under 20% sucrose+20% PEG4000+ 40 mg/L H3BO3+3×10-3 mol/L Ca(NO3)2+10 mg/L VB1 at 28℃ for 30 min. Meanwhile, the identification results of the three series of wheat, including K-type wheat sterile line, maintainer line and F1 materials, showed that the medium was more suitable for in vitro pollen germination of wheat, and was able to accurately detect wheat pollen activity. At the same time, the study could provide certain theoretical basis for the preservation of wheat pollen and wheat breeding.