Abstract: Eotetranychus sexmaculatus (Riley) is an important mite pest of rubber tree in Xishuangbanna. To study its principal biological characteristics, developmental durations of Eotetranychus sexmaculatus (Riley) in different stages were studied under a range of constant temperatures. Developmental zero and effective accumulated temperature were calculated with linear models. The results indicated that the relationship between temperature and development rate, each stage fitted well when the temperature was from 18℃ to 30℃. The developmental zero for eggs was (12.32±1.33)℃ and the effective accumulated temperature for eggs was (62.52±6.69)℃·d. The developmental zero from larva to adult was (9.38±3.30)℃ and the effective accumulated temperature from larva to adult was (153.79±33.46)℃·d. The developmental zero of the whole generation was (9.52±1.70)℃ and the effective accumulated temperature of the whole generation was (233.18±26.25)℃·d. The results provided a scientific basis for monitoring and integrated management of Eotetranychus sexmaculatus (Riley).