Abstract: To investigate the effects of different growth temperature on photosynthesis, content of plastid pigments, enzyme activities and gene expression of plastid pigment catabolism in tobacco leaves at different growth stages, the tobacco cultivar‘Yunyan 87’was used as experimental material, and different growth temperatures were simulated in artificial climate chambers similar to the temperatures in Jiangchuan Yunnan, Zunyi Guizhou, and Xiangxian Henan during the transplanting to resettling stage, resettling to squaring stage and mature stage of‘Yunyan 87’plants. The results indicated that the growth temperature treatments simulated to the above- mentioned three regions had similar effects on photosynthetic rate, content of chlorophyll and carotenoid, and activities of metabolism related enzymes with prolonged tobacco growth and development stages, which firstly showed an lower level at early growth stage, then rise at middle growth stage, and gradually decline at mature stage, like a parabolic type. In general, different growth temperatures all could influence photosynthetic rate, content of plastid pigments, activities of metabolism related enzymes and gene expression to some extent in tobacco leaves at different growth stages. Compared to other growth stages, the temperature at mature stage demonstrated the most significant effects on photosynthetic rate and content of plastid pigments. Xiangxian, which had higher growth temperature than the other two regions, was more suitable for photosynthesis and accumulation of plastid pigments in tobacco leaves. In addition, the temperature simulated to Zunyi was also suitable for carotenoid accumulation. These results explain why tobacco leaves from these three regions have great differences in tobacco flavor style based on photosynthesis and plastid pigment catabolism to some extent.