Abstract: Wufanshu (Vaccinium bracteatum Thunb.) is an excellent rootstock for blueberry (Vaccinium spp.). Under natural condition, Wufanshu seeds have a very low germination rate. To investigate the effect of gibberellic acid (GA3) on seed germination, Wufanshu seeds were soaked in solutions containing nine different concentrations of GA3 and distilled water (control) for 24 hours, and then incubated at 25℃ for 24 days. The research showed that the peak of seed germination was from the 12th to the 18th day. More seeds germinated in the GA3-treated groups than in the control group from the 16th to the 24th day. On the 24th day, the highest proportion of germinated seeds (81.33%) was in the 800 mg/LGA3 treatment group (33% higher than that in the control group). The proportion of germinated seeds in the 100-600 mg/L GA3 treatment groups ranged from 73.33% to 76.17%, but was lower in the 50 and 1000 mg/L GA3 treatment groups (63.83% and 67.33%, respectively). The germination potential and indices of the 100-800 mg/L GA3 treatment groups, especially the 800 mg/L GA3 treatment group, were higher than those of the control group. These results showed that an 800 mg/L GA3 soaking treatment for 24 hours strongly promoted Wufanshu seed germination. This method may serve as a reference in the seed propagation of Wufanshu.