Abstract: Based on the fact that the compatibility of some current coating rate determination methods for reconstituted tobacco in tobacco industry are not very ideal, the authors studied the organic chemical composition and their distribution in coating liquor and finished good sheet (on total sugar, reduced sugar, total alkaloid, total nitrogen), conducted a variation analysis of the organic chemical composition after coating and drying, and researched the corresponding relationship between organic chemical composition and coating rate before and after drying. Then, the authors proposed a method to indicate coating rate of reconstituted tobacco product with organic chemical composition. The results indicated that there was positive linear relationship between coating rate and the content of total sugar, reduced sugar and total alkaloid when the coating rate of reconstituted tobacco products in the range of 38%-46%, linear regression analysis showed that their linear correlation coefficients in the fitting formula were 0.966, 0.997 and 0.953. Through online random sample inspection, it was found that the average deviation of the measured and calculated values of the coating rate was less than 2%, which proved that the off-line detection method was an accurate and feasible method to detect the coating rate of the finished reconstituted tobacco. The research provides basic references for reconstituted tobacco industry and tobacco companies in the research of coating rate detection method, especially the off-line detection method for reconstituted tobacco products.