Abstract: The study aims to investigate the effects of genotype and environment interaction on yield stability and site discrimination of Job’s Tears in China, and provide guidance for identifying, recommending and registering excellent cultivars in production scientifically. The combination of double mark figure and special interaction [Dg(e)] of AMMI model was used to identify yield stability and site discrimination of the Job’s Tears cultivars, whose data came from the national regional trials of 2012-2014. The results showed that the output of 6 varieties ranged from 2380.0- 6061.0 kg/hm2, 1933.3- 5790.0 kg/hm2, 2192.0- 5632.3 kg/hm2, 905.3-5485.7 kg/hm2, 978.3-4680.0 kg/hm2, 991.0-5340.0 kg/hm2, respectively. There were significant effects of genotype (G), environment (E) and G×E interaction. The percentage of the sum of squares of genotype, environment and G × E interactive effects on total sum of squares were 8.41% , 55.10% and 14.03% , respectively. IPCA1, IPCA2 and IPCA3 represented the 60.97%, 18.97% and 3.07% of G?E interaction with a total of 83.01%, of which the IPCA1 was at 0.01 significant level. Therefore,‘Qianyijian3’,‘Anziyiyi’and ‘Wenyi2’had higher and more stable output, which should be recommended.‘Puyi6’had a moderate output,