Antioxidant Activity in vitro of the Ethylacetate Fraction from Aegiceras corniculatum Fruit

doi:10.11924/j.issn.1000-6850.casb15060054

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (28): 13-17.doi: 10.11924/j.issn.1000-6850.casb15060054

Special Issue: 园艺

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Antioxidant Activity in vitro of the Ethylacetate Fraction from Aegiceras corniculatum Fruit

Cai Jianxiu¹, Gu Yaqing¹, Huang Xiaodong^1,2, Huang Jianxin³, Li Yuanyue², Zhu Xiao¹

(¹Fujian Advanced Education Key Laboratory of Inshore Resources Biotechnology, Quanzhou Normal University, Quanzhou Fujian 362000;²Fujian Provincial Key Laboratory of Marine Resources and Eco-environment, Jimei Fujian 361000;³The Second Affiliated Hospital of Fujian Medical University, Quanzhou Fujian 362000)

Received:2015-06-09 Revised:2015-07-21 Accepted:2015-07-24 Online:2015-10-27 Published:2015-10-27

Abstract

Abstract: To study the antioxidation of the ethylacetate fraction from Aegiceras corniculatum fruit, the scavenging effects of ethylacetate fraction on DPPH and hydroxyl radical were investigated in vitro, total reducing power was determined. The inhibiting effects of ethylacetate fraction on mice RBC (the red blood cell) hemolysis induced by H₂O₂ and on MDA (malondialdehyde) formation of mice liver homogenate were also examined. The results were as follows: the clearance rate of DPPH and hydroxyl radical of the ethylacetate fraction from Aegiceras corniculatum fruit increased with its mass concentration increasing. Under the concentration of 0.01, 0.05, 0.1 mg/mL, the inhibitory rate of ethylacetate fraction on mice RBC hemolysis by H₂O₂inducement was 47.64%, 67.09%, 81.99%, respectively, and under the concentration from 0.5 mg/mL to 2.0 mg/mL, the ethylacetate fraction inhibited MDA production of mice liver homogenate very significantly (P< 0.01) compared with negative control group. Thus, it was clear that the ethylacetate fraction from Aegiceras corniculatum fruit had obvious antioxidant activity.

Cai Jianxiu,Gu Yaqing,Huang Xiaodong,Huang Jianxin,Li Yuanyue and Zhu Xiao. Antioxidant Activity in vitro of the Ethylacetate Fraction from Aegiceras corniculatum Fruit[J]. Chinese Agricultural Science Bulletin, 2015, 31(28): 13-17.

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Antioxidant Activity in vitro of the Ethylacetate Fraction from Aegiceras corniculatum Fruit

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