Abstract: Taking tomato ‘Fengshou’ as test material, an experiment was carried out with a system of enclosed inorganic substrate intelligent circulation trough supplying nutrition cyclically, in order to explore the effects of different amounts of nutrient supplying per plant in a certain period of time on greenhouse tomato growth, yield and quality. The results showed that T₃ (amount of nutrient supplying was 4.5 L/plant) had the highest organic acid and soluble sugar content, and the difference among the other 5 treatments had statistical significance. T₄ (amount of nutrient supplying was 6.0 L/plant) had higher plant height, weight per fruit, yield, soluble solids and Vc content, the difference of Vc content was more obvious, soluble solids had small differences among the treatments. When tomatoes grew 135 days, plant height of T₄ was 24.4% higher than that of T₆, which had the lowest plant height. In the whole fruit growth stage, the yield and weight per fruit of T₄ was 29.0% and 9.5% higher than that of T₁. Vc and soluble solids content of T₄ were 34.3% and 10% higher than that of T₁, the organic acid and soluble sugar content of T₃ were 17.8% and 27.1% higher compared with that of T₁. As a result, more and less amount of nutrient supplying was not conducive to increase the yield and improve the quality of greenhouse tomato. Both the yield and quality of greenhouse circulation trough tomato were better when the amount of nutrient supplying was kept between 4.5 to 6.0 L/plant in two hours.