Abstract: In order to clarify the allelic variation of polyphenol oxidase (PPO) in Shanxi wheat varieties (lines), and select and breed the wheat varieties with low activity of PPO, a total of 121 Shanxi wheat varieties (lines) were tested with the functional markers PPO16, PPO18 and PPO29 of PPO. The results showed that the wheat varieties (lines) with Ppo- A1a genotype and Ppo- A1b had 56 and 65 accessions, respectively, and the frequencies were 46.3% and 53.7%, respectively. The wheat varieties (lines) with Ppo-D1a genotype and Ppo-D1b had 50 and 71 accessions, respectively, and the frequencies were 41.3% and 58.7%, respectively. The wheat varieties (lines) with two-genotype combination types Ppo-A1a/Ppo-D1a, Ppo-A1a/Ppo-D1b, Ppo-A1b/ Ppo-D1a, Ppo-A1b/Ppo-D1b had 43, 50, 37, 56 accessions, respectively, and the frequency distributions were 35.5%, 41.3%, 30.6% and 46.3%, respectively. 37 accessions of the wheat varieties with low activity of PPO (Ppo-A1b/Ppo-D1a) were detected, which provided the basis for obtaining the wheat varieties with low activity of PPO in conventional breeding.