Abstract: The paper aims to study the effect of different plant growth regulators on redundant vegetative shoots growth and yield characteristics of cotton and explore new technology for inhibiting redundant vegetative shoots growth and simplifying the management of pruning in large scale cotton cultivation. The contrast test under field condition was conducted with three plant growth regulators (Duolingzhuanjia, AFD and DPC) to study the effect of different plant growth regulators on plant morphology, redundant vegetative shoots growth, spatial distribution of bolls and yield. The results showed that the inhibition effects of Duolingzhuanjia and AFD on average length of the upper main stem and the middle-upper fruit branch were weaker than that of DPC. The dry and fresh weight of redundant vegetative shoot per plant under Duolingzhuanjia and AFD treatments were significantly less than that under DPC treatment. Compared to DPC treatment, the fresh weight of redundant vegetative shoot per plant under Duolingzhuanjia and AFD treatments were 54.8% and 49.4% of that of the DPC treatment , the dry weight were 53.2% and 54.8% of that of the DPC treatment, the total length were 69.0% and 58.1% of that of the DPC treatment, the bolls per plant were 7% and 12.3% higher than that of the DPC treatment, and the yield were 12.6% and 10.2% higher than that of the DPC treatment. Duolingzhuanjia could increase internal bolls of plant and AFD could increase external bolls, they all had a trend for increasing the small bolls of main stem leaf axils.