Inhibitory Effect of the Methanol Extracts from Ishige okamurai Yendo on Tyrosinase

doi:10.11924/j.issn.1000-6850.casb15070068

Abstract

Abstract: To study the inhibitory effect of the extract from Ishige okamurai Yendo on tyrosinase, inhibition ratio of the methanol extracts from Ishige okamurai on the activity of tyrosinase was researched by the method of oxidation rate of L- DOPA, and its inhibition kinetic type was inferred by enzymatic dynamic test and Lineweaver-Burk curve method. The content of total phenols, polysaccharide and flavonoids was determined by Folin-Ciocalteu method, phenol-sulfuric acid method and AlCl3 color-developing method, respectively. The results showed that the methanol extracts from Ishige okamurai could obviously inhibited activity of tyrosinase with IC50 being 1.823 g/L. The inhibition kinetic characteristics were in accord with the noncompetitive inhibition. Chemical analysis showed that the methanol extracts had high content of total phenol, polysaccharides and flavonoids, which was (51.37 ± 2.77), (35.92 ± 5.27), (43.16 ± 0.33) mg/g, respectively.

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References

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[38]商飞.羽藻和缘管浒苔提取物对酪氨酸酶活性抑制作用的研究[D].中国海洋大学,2011.Tyrosinase Inhibitory Effect of the Methanol Extracts from Seaweed,Ishige okamurai YendoCHEN Jingming HUANG Xiaodong CAI Jianxiu(1.Quanzhou Preschool Education College, Quanzhou, Fujian, 362000,China；2. College of Chemistry and Life Science,Quanzhou NormalUniversity,Quanzhou, Fujian, 362000,China)Abstract:Inhibition effect of the methanol extracts from Ishige okamurai on tyrosinase activity was researched by the method of oxidation rate of L-dopamine,and its inhibition kinetic type was infered by enzymatic dynamic test and Lineweaver-Burk curve method.The content of total phenols, polysaccharide and flavonoids were determined by Folin-Ciocalteu method, phenol-sulfuric acid method and AlCl3 method ,respectively. Results show that the the methanol extracts from Ishige okamurai appears obvious tyrosinase inhibitory activity ,its IC50 is 1.823 g/L.,its inhibition kinetic characteristics is accord with the noncompetitive type inhibition. Chemical analysis find that the methanol extracts have high content of total phenol, polysaccharides and flavonoids,with the content of (51.37±2.77)mg/g,(35.92±5.27)mg/g,(43.16±0.33)mg/g,respectively.Keywords:Ishige okamurai Yendo, methanol extract;tyrosinase;inhibition kinetics