Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (1): 107-112.doi: 10.11924/j.issn.1000-6850.casb15080023

Special Issue: 生物技术

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DNA Fingerprinting of 40 Dimocarpus longan Germplasms by ISSR Markers and Analysis of Genetic Relationship

  

  • Received:2015-08-05 Revised:2015-09-16 Accepted:2015-09-25 Online:2016-01-28 Published:2016-01-28

Abstract: To differentiate and identify varieties and types of Dimocarpus longan germplasm, the inter-simple sequence repeat (ISSR) method was applied to assess the genetic variations of 40 Dimocarpus longan varieties including 4 new varieties. From 80 ISSR primers, 11 were employed on 40 samples. Cluster analysis revealed that the genetic similarity coefficient ranged from 0.58 to 0.88, and the 40 varieties could be clustered into 3 groups using a genetic similarity coefficient threshold of 0.64. The 40 varieties could be distinguished completely by 2 ISSR primers (UBC810/UBC818), and digital DNA fingerprint was established accordingly. This study provided an important basis for the identification of new varieties, and the evaluation and utilization of germplasm resources.

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