Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (3): 79-85.doi: 10.11924/j.issn.1000-6850.casb15080067

Special Issue: 资源与环境

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Genetic Diversity Analysis of Taraxacum kok-saghyz Rodin Germplasm by SSR Markers

张继川,张立群,甘霖,覃 碧 and 刘实忠   

  • Received:2015-08-12 Revised:2015-12-24 Accepted:2015-10-23 Online:2016-01-29 Published:2016-01-29

Abstract: Taraxacum kok-saghyz Rodin is an important rubber producing crops emerging in recent years with high quality rubber in the root.In order to know the genetic background and diversity of Taraxacum kok-saghyz Rodin germplasm collected. 23 pairs of SSR markers were used to analysis the genetic diversity of 96 accessions of Taraxacum kok-saghyz Rodin germplasm. Using 23 pairs of SSR markers, 71 polymorphic SSR loci were detected, 2 to 6 alleles were detected by each pair of primer, with an average of 3.09. Based on UPGMA clustering results, 96 accessions were divided into 2 groups. Group I contained all the Russian and American accessions, and 5 Xinjiang wild accessions, Group II contained the rest of Xinjiang wild accessions from 7 populations. Russian and American accessions belonged to subgroup A, the average genetic similarity coefficient among them was 0.88, which suggested that close genetic relationship was exited among them. 7 Xinjiang populations were divided into 5 subgroups, which showed wide genetic polymorphisms and complex genetic relationships among them. The analysis results of genetic diversity proved that, SSR marker could be used for genetic diversity research effectively which will provide the theorectic and practic base for germplasm collection and breeding of Taraxacum kok-saghyz Rodin in the future.

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