Abstract: To evaluate the toxicity of common biological insecticides and provide theoretical basis for effective control of aphids on Chinese medicinal herb, we conducted toxicity test of 4 biological insecticides (Avermectins, Azadirachtin, Spinetoram and Matrine) to 3 aphids (Semiaphis heraclei, Myzus persicae and Uroleucon gobonis) by dipping method in the laboratory. The results showed that Avermectins was the best to control 3 aphids (LC50 was 9.125 mg/L, 0.730 mg/L and 1.135 mg/L, respectively), followed by Azadirachtin (LC50 was 103.425 mg/L, 2.318 mg/L and 2.483 mg/L, respectively). Meanwhile, Spinetoram and Matrine showed the lowest toxic activity. Therefore, Avermectins and Azadirachtin were excellent pesticides to control 3 aphids on Chinese medicinal herb, and could be popularized and applied after field experiment.