Optimization of Poly (Vinyl Alcohol)-Degrading Enzyme Production Condition in Bacillus amyloliquefaciens HK1

doi:10.11924/j.issn.1000-6850.casb15090011

Abstract

Abstract: The strain HK1 was isolated from environment, which could degrade polyvinyl alcohol. Using the I₂-KI and boric acid staining method, it was proved that HK1 had intracellular and extracellular degrading enzymes, mainly extracellular enzymes. Optimization of culture conditions was carried out through mono-factor and orthogonal experiments. The results showed that: the optimum loading volume, inoculum density and culture temperature was 50 mL/250 mL, 6% and 30℃, respectively. The optimum external carbon source and nitrogen source was PVA and NH₄NO₃, respectively. The optimal fermentation condition was: PVA 2.5 g/L and NH₄NO₃ 0.6 g/Lat pH 7.0. Under the optimal fermentation condition, the production of degrading enzyme of strain HK1 was 225% higher than when it was cultured in former fermentation condition.

CLC Number:

X172

Song Yaheng,Zhou Yunheng,Li Sujian,Liu Yunke and Wei Yahong. Optimization of Poly (Vinyl Alcohol)-Degrading Enzyme Production Condition in Bacillus amyloliquefaciens HK1[J]. Chinese Agricultural Science Bulletin, 2016, 32(2): 33-39.

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