Abstract: [Objective] Analysised the effect of different carbon source on amino acids and various amino acid quantitative traits,in order to optimize the pleurotus ostreatus culture material to provide a scientific basis. [Method]The correlation degree of quantitative traits of amino acids and different amino acids with different kinds of carbon source by grey correlation analysis. [Result]The characters of amino acids on pleurotus ostreatus by different carbon source in the order of correlation: Brown sugar > lactose >Sugar > Soluble starch, the correlation degree of brown sugar treatment was 0.6127, the Soluble starch treatment was 0.5057;The characters of various amino acids on pleurotus ostreatus with different carbon source in the order of correlation: Brown sugar >Sugar >lactose >Soluble starch, The correlation degree of brown sugar treatment was 0.7654, Soluble starch treatment was 0.5085. [Conclusion]The bigger degree of connection is the better effect on pleurotus ostreatus amino acids, indicating the influence in the quantity and quality of amino acids on Pleurotus ostreatus by carbon source. Brown sugar was the best carbon source in the culture medium of pleurotus ostreatus.